# Transitions and transversions in R

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A couple of months ago I wrote the following R function to calculate the number of transitions and transversions between DNA sequences in an alignment. The function is fairly slow (an alignment of ~100 sequences, 800 bp in length takes around 30 seconds to run) thanks to the double for loop, however in this case I shall plead Uwe’s Maxim: “Computers are cheap and thinking hurts”.**The Praise of Insects**, and kindly contributed to R-bloggers]. (You can report issue about the content on this page here)Want to share your content on R-bloggers? click here if you have a blog, or here if you don't.

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library(ape)

#Input: dat—an object of class ‘DNAbin’

titv<-function(dat){

mat<-as.matrix(dat)

res<-matrix(NA, ncol=dim(mat)[1], nrow=dim(mat)[1], dimnames=list(x=names(dat), y=names(dat)))

for(i in 1:dim(mat)[1]){

for(j in 1:dim(mat)[1]){

vec<-as.numeric(mat[i,])+as.numeric(mat[j,])-8

res[i,j]<-length(grep("200|56",vec)) #Transitions

res[j,i]<-length(grep("152|168|88|104",vec)) #Transversions

}

}

res

}

#Example

data(woodmouse)

ti<-titv(woodmouse)

tv<-t(ti)

tv[lower.tri(tv)] #Number of transversions

ti[lower.tri(ti)] #Number of transitions

#Saturation plot

dist<-dist.dna(woodmouse)

plot(ti[lower.tri(ti)]~dist)

points(tv[lower.tri(tv)]~dist, pch=20, col=”red”)

To

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