Blog Archives

Pathway Analysis for High-Throughput Genomics Studies

March 6, 2012
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Pathway Analysis for High-Throughput Genomics Studies

I get a lot of requests in the core about running a "pathway analysis." Someone ran a handful of gene expression arrays, or better yet, ran an RNA-seq experiment (with replicates!). These, and many other kinds of high-throughput assays (GWAS, ChIP-seq,...

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I’m Hiring!

February 24, 2012
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I direct the Bioinformatics Core at the University of Virginia, and I'm hiring. Visit this link on the UVA Jobs website for more information. Here's the description:The University of Virginia Bioinformatics Core is seeking a full-time position as a bio...

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Hadley Wickham: ggplot2 Webinar (Today!)

February 8, 2012
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Title: A Backstage Tour of ggplot2 with Hadley WickhamDate: Wednesday, February 8, 2012Time: 11:00AM - 12:00PM PacificPresenter: Hadley Wickham, Professor of Statistics, Rice UniversityRegister here.I used ggplot2 extensively a few years ago, but rever...

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Annotating limma Results with Gene Names for Affy Microarrays

January 17, 2012
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Annotating limma Results with Gene Names for Affy Microarrays

Lately I've been using the limma package often for analyzing microarray data. When I read in Affy CEL files using ReadAffy(), the resulting ExpressionSet won't contain any featureData annotation. Consequentially, when I run topTable to get a list of di...

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New Year’s Resolution: Learn How to Code

January 5, 2012
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Farhad Manjoo at Slate has a good article on why you need to learn how to program. Chances are, if you're reading this post here you're already fairly adept at some form of programming. But if you're not, you should give it some serious thought.Gina Trapani, former editor of tech blog Lifehacker, is quoted in the article:“Learning...

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Query a MySQL Database from R using RMySQL

December 15, 2011
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I use this all the time, and the setup is dead simple. Follow the code below to load the RMySQL package, connect to a database (here the UCSC genome browser's public MySQL instance), set up a function to make querying easier, and query the database to ...

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RNA-Seq & ChiP-Seq Data Analysis Course at EBI

December 8, 2011
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I just got this announcement from EMBL-EBI about an RNA-seq/ChIP-seq analysis hands-on course. Find the full details, schedule, and speaker list here.Title: Advanced RNA-Seq and Chip-Seq Data Analysis Course Date: May 1-4 2012 Venue: EMBL-EBI, Hinxton,...

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An example RNA-Seq Quality Control and Analysis Workflow

December 6, 2011
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I found the slides below on the education page from Bioinformatics & Research Computing at the Whitehead Institute. The first set (PDF) gives an overview of the methods and software available for quality assessment of microarray and RNA-seq experim...

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GEO2R: Web App to Analyze Gene Expression in GEO Datasets Using R

November 17, 2011
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GEO2R: Web App to Analyze Gene Expression in GEO Datasets Using R

Gene Expression Omnibus is NCBI's repository for publicly available gene expression data with thousands of datasets having over 600,000 samples with array or sequencing data. You can download data from GEO using FTP, or download and load the data direc...

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I’m Starting a New Position at the University of Virginia

September 8, 2011
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I’m Starting a New Position at the University of Virginia

I just accepted an offer for a faculty position at the University of Virginia in the Center for Public Health Genomics / Department of Public Health Sciences. Starting in October I will be developing and directing a new centralized bioinformatics core ...

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