Blog Archives

Stepping Outside My Open-Source Comfort Zone: A First Look at Golden Helix SVS

May 16, 2012
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Stepping Outside My Open-Source Comfort Zone: A First Look at Golden Helix SVS

I'm a huge supporter of the Free and Open Source Software movement. I've written more about R than anything else on this blog, all the code I post here is free and open-source, and a while back I invited you to steal this blog under a cc-by-sa license....

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NSF BIGDATA webinar

May 1, 2012
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If you're doing any kind of big data analysis - genomics, transcriptomics, proteomics, bioinformatics - then unless you've been on vacation the last few weeks you've no doubt heard about the NSF/NIH BIGDATA  Initiative (here's the NSF solicitation...

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RNA-Seq Methods & March Twitter Roundup

April 6, 2012
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There were lots of interesting developments this month that didn't work their way into a full blog post. Here is an incomplete list of what I've been tweeting about over the last few weeks. But first I want to draw your attention to the latest manuscri...

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Video Tip: Convert Gene IDs with Biomart

March 14, 2012
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I get asked frequently how to convert from one gene identifier to another. This can be tricky, especially when relying on gene symbols, as Will pointed out in a previous post a few years ago. There are several tools that can do this, including DAVID an...

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find | xargs … Like a Boss

March 9, 2012
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*Edit March 12* Be sure to look at the comments, especially the commentary on Hacker News - you can supercharge the find|xargs idea by using find|parallel instead.---Do you ever discover a trick to do something better, faster, or easier, and wish you c...

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Pathway Analysis for High-Throughput Genomics Studies

March 6, 2012
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Pathway Analysis for High-Throughput Genomics Studies

I get a lot of requests in the core about running a "pathway analysis." Someone ran a handful of gene expression arrays, or better yet, ran an RNA-seq experiment (with replicates!). These, and many other kinds of high-throughput assays (GWAS, ChIP-seq,...

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I’m Hiring!

February 24, 2012
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I direct the Bioinformatics Core at the University of Virginia, and I'm hiring. Visit this link on the UVA Jobs website for more information. Here's the description:The University of Virginia Bioinformatics Core is seeking a full-time position as a bio...

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Hadley Wickham: ggplot2 Webinar (Today!)

February 8, 2012
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Title: A Backstage Tour of ggplot2 with Hadley WickhamDate: Wednesday, February 8, 2012Time: 11:00AM - 12:00PM PacificPresenter: Hadley Wickham, Professor of Statistics, Rice UniversityRegister here.I used ggplot2 extensively a few years ago, but rever...

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Annotating limma Results with Gene Names for Affy Microarrays

January 17, 2012
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Annotating limma Results with Gene Names for Affy Microarrays

Lately I've been using the limma package often for analyzing microarray data. When I read in Affy CEL files using ReadAffy(), the resulting ExpressionSet won't contain any featureData annotation. Consequentially, when I run topTable to get a list of di...

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New Year’s Resolution: Learn How to Code

January 5, 2012
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Farhad Manjoo at Slate has a good article on why you need to learn how to program. Chances are, if you're reading this post here you're already fairly adept at some form of programming. But if you're not, you should give it some serious thought.Gina Trapani, former editor of tech blog Lifehacker, is quoted in the article:“Learning...

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