A short post to announce TrackMateR, a new R package to analyse TrackMate XML outputs.

• Code
• Instructions

Background

TrackMate is a plug-in for ImageJ which ships with Fiji. It’s essential for single particle tracking work, particularly for microscopy movies. For example, tracking the movement of fluorescent vesicles inside cells.

A tracking session generates a TrackMate XML file. The idea was to write something for R that could load these XML files and do some analysis.

Features

The package has functions to display tracks and to analyse:

• speed, displacement, cumulative distance
• mean squared displacement, alpha
• jump distance
• fractal dimension
• track density (number of neighbouring tracks with a search radius)

Usage

Full instructions are here. Briefly, install via devtools.

# install.packages("devtools")
devtools::install_github("quantixed/TrackMateR")

The idea is that a user would have either one TrackMate file to analyse or many.

One TrackMate file

The user can load and process the file automatically to generate a report:

library(ggplot2)
library(TrackMateR)
# an example file is provided, otherwise use file.choose()
xmlPath <- system.file("extdata", "ExampleTrackMateData.xml", package="TrackMateR")
# read the TrackMate XML file into R using
tmObj <- readTrackMateXML(XMLpath = xmlPath)
# Pixel size is 0.04 um and original data was 1 pixel, xyscalar = 0.04
tmObj <- correctTrackMateData(dataList = tmObj, xyscalar = 0.04, xyunit = "um")
# automatically generate report using
reportDataset(tmObj)

or they can fine tune the parameters to generate a report using different settings (details here).

Multiple TrackMate files

Using compareDatasets() it is possible to analyse multiple datasets using different conditions. This workflow will generate:

• one report per dataset
• one summary per condition
• one comparison of all conditions
• a number of text file outputs for reuse

Thanks

The code builds on initial work on a package called TrackR from Julien Godet. Some test data generated by Méghane Sittewelle is included to get started.

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The post title comes from “Tracking” by Ambush which comes from a compilation “Sonics Everywhere”