I’m working on a project using next-gen sequencing to fine-map a genetic association in a gene region. Now that I’ve sequenced the region in a small sample, I’m picking SNPs to genotype in a larger sample. When designing the genotyping assay the lab will need flanking sequence.
This is easy to get for SNPs in dbSNP, but what about novel SNPs? Specifically, given a list of genomic positions where