DNA methylation (RRBS or target capture) analysis with R

February 20, 2012

(This article was first published on Recipes, scripts and genomics, and kindly contributed to R-bloggers)

Reduced Representation Bisulfite sequencing (RRBS) is a popular technique for measuring methylation levels across genome. Although it does not have the full genome coverage, it covers many important regions for methylation. Below, I shared a tutorial and slides on how to do basic RRBS analysis using R and methylKit package. The same methods can also be used on the aligned reads obtained from a target-capture protocol such as Agilent SureSelect Methyl-Seq.

The tutorial includes sections such as clustering samples and doing differential methylation analysis, and some R basics.

The tutorial is here:

The tutorial slides are below:

The slides are also downloadable from here:

To leave a comment for the author, please follow the link and comment on their blog: Recipes, scripts and genomics.

R-bloggers.com offers daily e-mail updates about R news and tutorials on topics such as: Data science, Big Data, R jobs, visualization (ggplot2, Boxplots, maps, animation), programming (RStudio, Sweave, LaTeX, SQL, Eclipse, git, hadoop, Web Scraping) statistics (regression, PCA, time series, trading) and more...

If you got this far, why not subscribe for updates from the site? Choose your flavor: e-mail, twitter, RSS, or facebook...

Comments are closed.


Never miss an update!
Subscribe to R-bloggers to receive
e-mails with the latest R posts.
(You will not see this message again.)

Click here to close (This popup will not appear again)