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Reduced Representation Bisulfite sequencing (RRBS) is a popular technique for measuring methylation levels across genome. Although it does not have the full genome coverage, it covers many important regions for methylation. Below, I shared a tutorial and slides on how to do basic RRBS analysis using R and methylKit package. The same methods can also be used on the aligned reads obtained from a target-capture protocol such as Agilent SureSelect Methyl-Seq.Want to share your content on R-bloggers? click here if you have a blog, or here if you don't.
The tutorial includes sections such as clustering samples and doing differential methylation analysis, and some R basics.
The tutorial is here:
http://methylkit.googlecode.com/files/methylKitTutorial_feb2012.pdf
The tutorial slides are below:
Analyzing RRBS methylation data < embed name="__sse11681735" src="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=methylkittutorialslidesfeb2012-120220193226-phpapp01&stripped_title=analyzing-rrbs-methylation-data&userName=altunaakalin" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" wmode="transparent" width="425" height="355">
View more presentations from Altuna Akalin.
The slides are also downloadable from here:
http://methylkit.googlecode.com/files/methylKitTutorialSlides_feb2012.pdf
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